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( D ) IRI protocol of retrograde, nonrecirculating Langendorff perfusion of isolated hearts from male 10- to 12-week-old WT or Glp1r / mice

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Detection of GSTP1/2, NRF2 and MnSOD were performed on paraffin sections by using anti-GSTpi (PB13020, Abnova, Tebu Bio Sas, Le Perray en Yvelines, France), anti-NRF2 (sc-722, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-MnSOD (D3X8F, Cell Signaling Technology, Beverly, MA, USA), respectively